![]() The plugins are organized in submenus, and the bundle is described in an extensively illustrated online manual (which evolved from the original lab instructions). Here the package was resurrected as MBF ImageJ, containing all the plugins that I have found useful. When I recently joined the McMaster Biophotonics Facility (MBF at McMaster University, Hamilton, Canada, I was encouraged to maintain this ImageJ for Microscopy bundle. Initially collated from the ImageJ home page to help the Laboratory of Molecular Signaling in Babraham Institute (UK), I developed it further at the Wright Cell Imaging Facility (TWRI, Canada) here it was released as WCIF ImageJ. The ImageJ for Microscopy bundle and accompanying manual was developed to manage this wide-ranging array of plugins. Once saved to the ImageJ plugins folder, these functions are loaded on start-up and can be accessed via menu commands like any other core function. These additional files are either written in Java (the plugins) or in ImageJ's macro programming language (macros). While Rasband is the author of the core program, an extensive group of additional developers has written and made available a growing arsenal of short add-on programs to provide additional functionality to the core program. ![]() However, a few steps into ImageJ, and this minor inconvenience is forgotten. One of the downsides of the Java heritage is an interface that may feel a little unfamiliar. The new 64-bit operating systems and their JRE have happily broken the long-held 1.7 Gb memory limit for Java applications. With JRE available for most operating systems, ImageJ is platform-independent, running on Macintosh, Windows, Linux, and even a PDA operating system. Java runtime environments (JRE) are freely available, either from Sun or bundled with platform-specific installations of ImageJ ( /ij). To run ImageJ, a given system needs only the operating system-specific Java runtime environment. By shifting to Java, Rasband liberated the software from an individual operating system. ![]() Wayne Rasband is the core author of ImageJ after developing the Macintosh-based National Institutes of Health (NIH) Image for 10 years, he made the brave decision of starting afresh with ImageJ using the Java programming language. In addition to its impressive functionality, this cutting-edge image-processing tool has an indispensable support community of enthusiasts on the ImageJ mailing list. ![]() These past 10 years have seen the Java-based open-source software mature into an invaluable laboratory tool. ImageJ will celebrate its tenth anniversary in September of this year. ![]()
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